Modifying Enzymes
ABclonal Technology T7 Endonuclease I
T7 Endonuclease I (T7 Endo I, T7EI)，can recognize and cleave non-perfectly mismatched DNA, cruciform DNA structures, holliday structures or junctions and Heteroduplex DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5? end to the mismatch.
IMMUNOLOGY CONSULTANTS Lysosomal Acid Lipase (Host Cell Protein) ELISA Kit – CHO (Chinese Hamster Ovary)
The Lysosomal Acid Lipase (Host Cell Protein) ELISA Kit – CHO (Chinese Hamster Ovary) is a highly sensitive immunoassay designed to detect Lysosomal Acid Lipase (LAL), a lipolytic enzyme involved in the breakdown of cholesteryl esters and triglycerides within lysosomes. As a residual host cell protein released from CHO cells during biologics manufacturing, LAL may contribute to polysorbate degradation and product stability risks if not effectively removed during purification.This assay enables precise quantification of residual LAL in bioprocess samples and supports manufacturers seeking greater visibility into high-risk lipase contaminants. This kit is ideal for HCP monitoring, bioprocess development, and biopharmaceutical quality control, helping manufacturers better detect problematic lipases that may impact formulation stability and product quality.
MERIDIAN LIFE SCIENCE Gly-Free T4 DNA Ligase -HC, 200UL
Glycerol-Free T4 DNA Ligase (HC) a glycerol-free, high concentration enzyme (50U/uL) that catalyzes the formation of a phosphodiester bond between adjacent 5ʹ phosphate and 3′ hydroxyl groups in duplex DNA or RNA. The enzyme efficiently joins blunt and sticky-ended fragments in just 15 and 10 minutes respectively at room temperature (25C). It can be used to perform linker ligation, re-ligation of linearized plasmids and ligation of double-stranded oligonucleotides into vectors and enables rapid ligation of up to 100ng of fragments from most sources including PCR fragments, plasmids, cosmids, genomic, phage and viral DNA into prokaryotic or eukaryotic plasmid vectors and bacteriophage lambda vectors.
ALKALI SCIENTIFIC INC Radiant™ HiFi Pro™ Hot Start Enzyme, 2u/ul with 5X Buffer, 500 Reactions, with Enhancer Juice
Radiant™ HiFi Pro™ Hot Start Enzyme, 2u/ul with 5X Buffer, 500 Reactions, with Enhancer Juice, is a versatile enzyme solution for reliable and high-fidelity PCR. With enhanced amplification performance, it ensures accurate and reproducible results across a wide range of templates. The 5X buffer and enhancer juice combination improves reaction efficiency, making it suitable for complex or challenging DNA amplification tasks. This enzyme is the go-to choice for researchers needing precise PCR results.
ILEX LIFE SCIENCES LLC Provis Biolabs PNGase F Recombinant E coli expressed Elizabethkingia miricola enzyme 100 U 100 UN 1/EA
Provis Biolabs PNGase F Recombinant (cloned from Elizabethkingia miricola) RUO Grade 100 U DESCRIPTION PNGase F is an endoglycosidase that removes N-linked glycan oligosaccharides from glycoproteins Manufactured under ISO 9001 2015 Animal-origin-free (AOF) Research use only (RUO) GMP Grade is available upon request (made-to-order custom production) APPLICATION Glycoprotein analysis Protein structure determination Antibody deglycosylation Amino acids sequence determination X-Ray crystallography MALDI-TOF or other mass-spec techniques CAS 83534-39-8 SOURCE E coli recombinant (6xHis-tagged) MASS 36 kDa PURITY (SDS-PAGE) 95% FORMULATION 10 000 U/mL in 20 mM Tris 50 mM NaCl 5 mM EDTA (0 2 m filtered) ACTIVITY 1 U deglycosylation of 1 nmol RNase B/min at 37 C STORAGE 2-8 C MANUFACTURER Provis Biolabs ORIGIN India OTHER NAMES PNGase F enzyme PNGaseF Glycopeptide N-glycosidase N-glycanase N-Glycosidase F Peptide N-glycosidase EC 3 5 1 52
ILEX LIFE SCIENCES LLC Provis Biolabs PNGase F Recombinant E coli expressed Elizabethkingia miricola enzyme 500 U 500 UN 1/EA
Provis Biolabs PNGase F Recombinant (cloned from Elizabethkingia miricola) RUO Grade 500 U DESCRIPTION PNGase F is an endoglycosidase that removes N-linked glycan oligosaccharides from glycoproteins Manufactured under ISO 9001 2015 Animal-origin-free (AOF) Research use only (RUO) GMP Grade is available upon request (made-to-order custom production) APPLICATION Glycoprotein analysis Protein structure determination Antibody deglycosylation Amino acids sequence determination X-Ray crystallography MALDI-TOF or other mass-spec techniques CAS 83534-39-8 SOURCE E coli recombinant (6xHis-tagged) MASS 36 kDa PURITY (SDS-PAGE) 95% FORMULATION 10 000 U/mL in 20 mM Tris 50 mM NaCl 5 mM EDTA (0 2 m filtered) ACTIVITY 1 U deglycosylation of 1 nmol RNase B/min at 37 C STORAGE 2-8 C MANUFACTURER Provis Biolabs ORIGIN India OTHER NAMES PNGase F enzyme PNGaseF Glycopeptide N-glycosidase N-glycanase N-Glycosidase F Peptide N-glycosidase EC 3 5 1 52